فصلنامه پاتوبیولوژی مقایسه ای
سال دهم شماره 39 (بهار 1392)

Subclinical hypocalcemia in dairy cattle is decrease in plasma calcium to less than 7.5 mg / dl with no clinical signs. The aim of this study was to determine the incidence of post-partum subclinical hypocalcemia and it’s correlation with negative energy balance in suburban of Urmia, Iran. In this study blood sampling was done from 99 multi-parous cows (2 to 5 calving) during the first 48 hours after parturition (to evaluate serum calcium) and second week after parturition (to determine the levels of non-esterified fatty acids (NEFA)).198 blood samples were taken from 99 dairy cattle from 498 cattle population. The incidence rate of subclinical hypocalcaemia in this study was 18.2%. NEFA mean in cows with subclinical hypocalcaemia was significantly (p 0.7mmol / L) in cows with subclinical hypocalcaemia was six times higher than cows with normal serum calcium levels. The results showed the bad state of energy balance in dairy cows with subclinical hypocalcemia and probability of negative energy balance induced diseases such as ketosis. Subclinical hypocalcemia increases the sensitivity of dairy cows to mastitis, retained placenta, displaced abomasum, dystocia and ketosis. With attention to limited available information about this disease, further studies are required.

The diabetes type 2 is the most common and growing issue of the world health. The purpose of this study is to evaluate the effects of “Metformin” on glucose, lipid profiles and oxidative stress in the alloxan-induced diabetic rats. In this study 40 Wistar rats, were randomly separated into 4 equal groups, containing: healthy control group, diabetic group, metformin-controlled group, and diabetic rats treated with metformin. Diabetes was induced by single dose of intraperitoneal injection of 120 mg/kg Alloxan. The groups 3 and 4 were fed 150 mg/kg/day of metformin for a period of one month. At the end, blood of all the rats were collected and the serum levels of glucose, triglycerides, cholesterol, LDL, HDL, VLDL, lipid peroxidation products as malondialdehyde index, plus the superoxide dismutase enzymes, glutathione peroxidase, catalase and total antioxidant in blood were measured. Finally, the quantitative data obtainedwere analyzed by ANOVA and Tukey’s post test amongst the groups at p

This study was conducted to investigate the effect of protein hydrolysate on rainbow trout alevine survival after bacterial challenge. Six diets based on different levels of sardine (Sardinella gibossa) protein hydrolysate (SPH) and poultry by-products protein hydrolysate(PPH) (10% , 25% and 50%) were tested. After nutrition period, the gastrointestinal section was separated by the sterile method and content of total count and Lactobacillus bacteria was measured. Also 60 fish was challenged by the Aeromonas salmonicida for 24 hours. Intestinal bacterial flour was higher in the FPH 25% and 50% treatments in compare to 10% and control (p 0.05). Results showed that FPH had better effect than PPH and this two protein hydrolysates in 25% replacement by fish protein in diet enhances the survival and non-specific immunity to Aeromonas salmonicida.

The objectives of the study were to validate the use of endometrial cytology to diagnose subclinical endometritis at 70 to 170 days in milk (DIM), in clinically normal postpartum dairy cows. The study population consisted of clinically normal Holstein-dairy cows at 70 to 170 daysin milk (DIM), and with no abnormal vaginal discharge based on visual inspection and vaginoscopy. Research procedure was done on 62 non-pregnant cows. On day 21 after a preenrollment AI, animals received 100 μg of GnRH, and then examined ultrasonographically 7 days later (day 28). An endometrial sample was collected by cytobrush technique in nonpregnant cows on day 28. These cows were inseminated using timed AI after the completion of the Ovsynch protocol 10 days after enrollment in the study. Pregnancy diagnosis was ultrasonographically done about 28 days after AI in Research group. The result of this study showed that the risk of nonpregnancy was higher in cows with ≥8% PMNs identified using cytobrush technique than in cows with

The aim of the present study was to evaluate the preventive effects of Turmeric (Curcuma Longa Linn.) powder on rat high fat diet-induced hepatic steatosis model. For this purpose, male wistar rats were treated in 4 experimental groups including: 1- healthy control group given standard diet, 2- high fat diet group for induction of hepatic steatosis, 3- high fat diet plus Clofibrate 320 mg/kg) as positive control, and 4- high fat diet plus Turmeric powder (5%) for protection of liver steatosis, at a period of 6 weeks. At the end of experiment, the groups were compared considering serum lipid profile, serum biomarkers of liver tissue injury and liver histopathological changes. After 6 weeks treatment, high fat diet caused significant (p

Infectious Bronchitis is an acute contagious Coronovirus infection in chicken flocks that causes upper respiratory, renal and reproductive diseases in infected premises. Detection and differentiation of causative agent is essential to plan prevention and control strategies such as vaccination. Because most of detection techniques are time consuming, a real-time polymerase chain reaction (RT-PCR) combined with high-resolution melt (HRM) curve analysis was developed based on the 3’UTR sequence of vaccinal strains as references of IBV toestablish a rapid detection and differentiation method for IBV strains. Results showed that the vaccinal reference strains were divided in four groups according to melt curve models for 400 to 430 bp PCR products and calculating Genotype Confidence Percentage (GCP). This Study resulted in an introduction of a geometric and arithmetic model for rapid and reliable classification of IBV strains base on 3’UTR HRM analysis.

Chickens may be infected with parasitic, viral and bacterial infections. The aim of this study was to determine the fauna, prevalence and severity of internal and external parasites of Chickens inIlam. In this study, 60 chickens were necropsied. Internal organs, feather and leg skin chips were examined. Feather, leg skin chips, gastrointestinal tract and femur muscle was infected withparasites. The identification of Nematodes was possible after they were cleared inlactophenol and it was possible to identify the cestoda after schneider acetocarmine staining. Isolated parasites from the feather, feathers follicle and leg skin chips were prepared and then examined. To distinguish blood and mucosal parasites, 2 smears and 1cc blood was taken from wing vein. Femur muscle was examined by dob smear while cecum, small and large intestine were smeared and then all were stained with geimsa. A smear from the trachea, trachea bifurcate, and the end of large intestine was prepared and then stained with Ziehl-Neelsen method. Prevalence and fauna of cestoda include: Raillietina echinobothrida 26.6%, Raillietina tetragona 26.6%, Raillietina cesticillus 13.3%, Choanotaenia infundibulum16.6%, Cotugnia digonopora 5. .Nematoda, Acuaria spiralis3.3%, Cheilospirura hamulosa 1.6%, Ascaridia galli 30% , Heterakis gallinarum 16.6%, and external parasites: Menopongallinae 55%, Goniodes spp. 18.3%, Lipeurus caponis 53.3% Menacanthus stramineus 58.3%, Goniocotes gallinae 81.6%, Cnemidocoptes mutans 23.3%, Grallobia spp. 33.3%. In non fecal protozoa, 1case of an unknown sarcocyct zoite was collected from femur muscle.